mRNA测序

特定组织或细胞在某一功能状态下转录的所有RNA的总和进行高通量测序。

产品介绍

转录组测序采用Illumina高通量测序平台,对人特定组织或细胞在某个特定状态下转录的所有mRNA进行测序,与参考基因组比对,既可全面快速分析mRNA序列和丰度信息、又可对基因结构和产生的新转录本进行分析。

应用方向
mRNA测序
技术优势
  • 无物种限制

无需了解物种的基因或基因组信息,便可直接对任意物种进行最全面的转录组分析。可检测未知基因,发现新的转录本。

  • 高精确度

数字化信号,可精确检测到基因家族中相似基因以及可变剪切造成的不同转录本的单碱基差异。

  • 高覆盖度

新一代高通量测序技术的高深度覆盖,可以检测到低丰度的稀有转录本。

项目流程
生信分析
mRNA_intermediate_workflow
技术参数
  • Total RNA:≥1ug,›50ng/ul
  • 测序策略:HiSeq X
  • 测序数据量:12G
  • 周期:30个工作日

 

样本类型 样品量
组织 100mg
细胞 1X106
全血 2ml
血清/血浆 1ml
结果展示
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