lncRNA测序

已有参考基因组物种的特定组织或细胞在某个特定时期转录出的所有lncRNA进行高通量测序

产品介绍

lncRNA测序采用Illumina HiSeq平台进行测序,针对有参考基因组样本开展准确的lncRNA鉴定和lncRNA靶基因预测,同时提供针对测序数据中mRNA的分析,结果更全面,广泛应用于医学、农学研究领域。

应用方向
lncRNA测序
技术优势
  • 高覆盖度

新一代高通量测序技术的高深度覆盖,可检测未知基因,发现新的转录本,可以检测到低丰度的稀有转录本

  • 与mRNA进行关联分析

可以将lncRNA与mRNA关联分析,挖掘lncRNA可能的功能信息

项目流程
生信分析
LncRNA_intermediate_workflow
技术参数
  • Total RNA:≥1ug,›50ng/ul
  • 测序策略:HiSeq X
  • 测序数据量:15G
  • 周期:30个工作日

 

样本类型 样品量
组织 100mg
细胞 1X106
全血 2ml
血清/血浆 1ml
结果展示
lncRNA部分合作文献

客户单位 期刊名称 文章标题 影响因子
1.第二军医大学(王品)2.浙江大学 SCIENCE An interferon-independent lncRNA promotes viral replication by modulating cellular metabolism 37.205
第二军医大 SCIENCE The STAT3-Binding Long Noncoding RNA lnc-DC Controls Human Dendritic Cell
Differentiation
37.205
第二军医大学医学遗传系 CANCER CELL A Long Noncoding RNA Activated by TGF-β Promotes theInvasion-Metastasis Cascade in Hepatocellular Carcinoma 23.523
1.中国科学院生物物理研究所,感染与免疫重点实验室2.解放军总医院肝胆外科 Cell Stem Cell The long noncoding RNA lncTCF7 promotes self-renewal of human liver cancer stem cells through activation of Wnt signaling. 22.387
第二军医大学分子遗传教研室 HEPATOLOGY LncRNA-LALR1 accelerates hepatocyte proliferation during liver regeneration by
activating Wntβ-Catenin signaling
11.055
第二军医大学 HEPATOLOGY Long Noncoding RNA High Expression in Hepatocellular Carcinoma Facilitates Tumor Growth Through Enhancer of Zeste Homolog 2 in Humans 11.055
复旦大学上海市肿瘤防治中心
乳腺外科。
Cancer Res Transcriptome analysis of triple-negative breast cancer reveals an integrated mRNA-lncRNA signature with predictive and prognostic value. 8.556
1.南京大学鼓楼医院2.南京大学模式动物研究中心3.中科院上海材料医学研究所 Theranostics TGFβ1 Promotes Gemcitabine Resistance through Regulating the LncRNA-LET/NF90/miR-145 Signaling Axis in Bladder Cancer 8.172
1.第二军医大学医学遗传中心2.第二军医大学长征医院3.南京大学金陵医院 Molecular Cancer Long noncoding RNA MRCCAT1 promotes metastasis of clear cell renal cell carcinoma via inhibiting NPR3 and activating p38-MAPK signaling 6.204
1.第三军医大学药学院转化医学中心,2.Riley心脏研究中心,美国印第安纳州印第安纳大学医学院儿科研究中心。3.苏州大学苏州血液与骨髓移植
研究所。4.美国俄亥俄州立大学哥伦布分校血液科,内科。
Sci Rep Profiling analysis of long non-coding RNAs in early postnatal mouse hearts. 5.228
上海交通大学仁济医院 JOURNAL OF
NEUROTRAUMA
Alteration in Long Non-Coding RNA Expression after Traumatic Brain Injury in Rats 5.19
第二军医大学长海医院 oncotarget Differentially expressed mRNAs, lncRNAs, and miRNAs with associated co-expression and ceRNA networks in ankylosing spondylitis 5.168
山东大学齐鲁医院2.山东大学医学院 Cell Physiol Biochem Huaier Extract Inhibits Breast Cancer Progression Through a LncRNA-H19_MiR
-675-5p Pathway
5.104
1.南京医科大学第四临床学院2.南京医科大学附属肿瘤医院胸外科,江苏省肿瘤研究所江苏省分子与转化生物学重点实验室 Oncotarget Comprehensive analysis of lncRNA expression profiles and identification of functional lncRNAs in lung adenocarcinoma. 5.008
1.大连医科大学口腔医学院口腔基础学院2.首都医科大学附属北京口腔医院 Stem Cell Res Ther Differential long noncoding RNA/mRNA expression profiling and functional
network analysis during osteogenic differentiation of human bone marrow
mesenchymal stem cells.
4.504
1.复旦大学2.南昌大学附属第二医院 Scientific Reports Long non-coding RNA00544 serves as a potential novel predictive and
prognostic marker for HR+ HER2-subtype breast cancer
4.259
1.第三军医大学药学院(邓有才)2.苏州大学 Scientific Reports Profiling analysis of long non-coding RNAs in early postnatal mouse hearts 4.259
1.第三军医大学生物医学工程学院(董世武)2.第三军医大学解剖系 Funct Integr Genomics Long noncoding RNA expression profiles in chondrogenic and hypertrophic
differentiation of mouse mesenchymal stem cells
3.496
山西医科大学第一医院 PLOS ONE Microarray Profiling and Co-Expression Network Analysis of Circulating lncRNAs
and mRNAs Associated with Major Depressive Disorder
3.234
首都医科大学 PLOS ONE The Noncoding RNA Expression Profile and the Effect of lncRNA AK126698 on
Cisplatin Resistance in Non-Small-Cell Lung Cancer Cell
3.234
第二军医大学附属长征医院 PLOS ONE Long noncoding RNA-EBIC promotes tumor cell invasion by binding to EZH2
and repressing E-cadherin in cervical cancer
3.234
西安交通大学第一附属医院新生儿科 Neuroscience Microarray Expression Profile Analysis of Long Noncoding RNAs in Premature
Brain Injury: A Novel Point of View.
3.231
第二军医大学附属长海医院神经外科 J CANCER RES CLIN LncRNA and mRNA interaction study based on transcriptome profiles reveals
potential core genes in the pathogenesis of human glioblastoma multiforme
3.081
中南大学 Toxicology in Vitro Overview of long non- coding RNA and mRNA expression in res ponse to methamphetamine treatment in vitro 2.866
首都医科大学公共卫生学院 EXP MOL PATHOL Long non-coding RNA NR_045623 and NR_028291 involved in benzene hematotoxicity in occupationally benzene-exposed workers 2.706
中国医科大学盛京医院 Oncol. Rep Microarray expression profile of long  non-coding RNAs in paclitaxel-resistant
human lung adenocarcinoma cells
2.662
1.首都医科大学附属北京天坛医院神经外科2.首都医科大学附属北京天坛医院临床医学核心实验室 Oncol. Rep Identification and functional characterization of lncRNAs acting as
ceRNA involved in the malignant progression of glioblastoma multiforme.
2.486
1.深圳疾控中心2.深圳先进技术研究院 Biochemical and
Biophysical Research
Communications
Long non-coding RNA expression profiles in different severity EV71-infected hand foot and mouth disease patients 2.466
1.首都医科大学 Neuroscience Letters Analysis of long non-coding RNA expression profiles following focal cerebral ischemia in mice 2.18
中国医学科学院北京协和医院妇产科,中国北京协和医科大学 Eur J Obstet Gynecol
Reprod Biol
Aberrantly expressed long noncoding RNAs in the eutopic endometria of
patients with uterine adenomyosis.
1.662
北京首都医科大学口腔学院 BIOMED RES INT Comparison of Long Noncoding RNA and mRNA Expression Profiles in
Mesenchymal Stem Cells Derived from Human Periodontal Ligament and Bone
Marrow
1.579
1.南方医科大学珠江医院2.温州医科大学附属第一医院 ONCOLOGY LETTERS Long non-coding RNA expression profile in cervical cancer tissues 1.39